![]() coli, goes through growth phases: infection, followed by multiplication, cell lysis and re-infection. Dust is phage heaven – they can survive for many years in a dry environment – so be thorough.Ĥ) Clean your pipettes with a strong disinfectant.ĥ) Put all your flasks (and all of your glassware, ideally) through the oven at 180☌.Ħ) Use new stoppers – the old wet ones (especially foam bungs) can be literally saturated with phage.ħ) Do not open your flasks during culture growth – you can control the air flow on your bench with a Bunsen burner, but it is never safe to open flasks for a “peek” while they are still in the incubator.īut what if contaminated culture is really unique and precious – the half-eaten colony is a clone you’ve been making for the last half a year or a strain you’ve been sent from a lab in Australia? Here are some tips for getting rid of phage:ġ) If you can throw out the contaminated culture, do it but don’t just put some vircon into it, autoclave the culture to kill the phage.Ģ) Discard all of the solutions you used to prepare the culture.ģ) Clean the shaker, your bench and other surfaces which might have contacted the contaminated culture, with ethanol. ![]() And it will not disappear unless you do something about it. Or you did a transformation, and the usually round colonies look like somebody has taken a bite out of them, and there are circular ‘no growth’ areas on the plate.Ĭondolences, you have a bacteria eater, aka: phage contamination. ![]() Your bacterial culture was growing calmly and predictably, dividing every twenty minutes, but suddenly its optical density has dropped, and it’s full of some sort of filaments and clumps. Your tissue culture has sprouted yeast contamination, your yeast culture is happily growing bacteria. Summertime… The birds are singing, the trees are growing.
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